Document Details

Document Type : Article In Journal 
Document Title :
Dissection of Hexosyl- and Sialyltransferase Domains in the Bifunctional Capsule Polymerases from Neisseria meningitidis Wand Y Defines a New Sialyltransferase Family
Dissection of Hexosyl- and Sialyltransferase Domains in the Bifunctional Capsule Polymerases from Neisseria meningitidis Wand Y Defines a New Sialyltransferase Family
 
Document Language : English 
Abstract : Background: Capsule polymerases of Neisseria meningitidis serogroups W and Y comprise hexosyl- and sialyltransferase activity. Results: Hexosyltransferase activity is encoded by the predicted N-terminal GT-B fold. Sialyltransferase activity requires 168 additional amino acids upstream of the predicted C-terminal GT-B fold. Conclusion: The sialyltransferase domains of NmW/Y define a new glycosyltransferase (CAZy) family. Significance: The new CAZy family comprises sequences from distantly related species. Crucial virulence determinants of disease causing Neisseria meningitidis species are their extracellular polysaccharide capsules. In the serogroups W and Y, these are heteropolymers of the repeating units (6)--d-Gal-(14)--Neu5Ac-(2)(n) in NmW and (6)--d-Glc-(14)--Neu5Ac-(2)(n) in NmY. The capsule polymerases, SiaD(W) and SiaD(Y), which synthesize these highly unusual polymers, are composed of two predicted GT-B fold domains separated by a large stretch of amino acids (aa 399-762). We recently showed that residues critical to the hexosyl- and sialyltransferase activity are found in the predicted N-terminal (aa 1-398) and C-terminal (aa 763-1037) GT-B fold domains, respectively. Here we use a mutational approach and synthetic fluorescent substrates to define the boundaries of the hexosyl- and sialyltransferase domains. Our results reveal that the active sialyltransferase domain extends well beyond the predicted C-terminal GT-B domain and defines a new glycosyltransferase family, GT97, in CAZy (Carbohydrate-Active enZYmes Database). 
ISSN : 0021-9258 
Journal Name : JOURNAL OF BIOLOGICAL CHEMISTRY 
Volume : 289 
Issue Number : 49 
Publishing Year : 1435 AH
2014 AD 
Article Type : Article 
Added Date : Thursday, August 10, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Angela RomanowRomanow, Angela Researcher  
Timothy KeysKeys, Timothy Researcher  
Katharina StummeyerStummeyer, Katharina Researcher  
Friedrich FreibergerFreiberger, Friedrich Researcher  
Bernard HenrissatHenrissat, Bernard Researcher  

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