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Document Details
Document Type
:
Thesis
Document Title
:
The Formation of Tetracycline in Date Medium by Mutants of Streptomyces aureofaciens Induced by some Chemical Mutagens
تكوين التتراسايكلين في بيئة التمر بواسطة طفرات من ستربتومايسيس أوروفاشينز مستحدثة ببعض المطفرات الكيميائية
Subject
:
Biological
Document Language
:
Arabic
Abstract
:
The present study deals with the genotoxicity of Nitrosoguanidine (N.T.G.) - a chemical mutagen- which was employed to induce auxotrophic mutations in the haploid wild type of bacteria Streptomyces aureofaciens strain NCIMB 8234. An increase in exposure time to the mutagen and dose of this mutagen led to a decrease in the survival percentage and increase in the percentage of mutation. The mutants were cultured in synthetic Date fermentation medium which contained Barni date-coat sugar extract as a carbon source. The following parameters were determined: final pH, medium quantity, microbial cell biomass to compare the changes before and after the incubation, and antibiotic production. All of the five mutants produced less antibiotic titers than the initial organism as certified by measuring the inhibition zone created by Staphylococcus aureus as a gram positive pathogenic bacterium and Escherichia coli as a gram negative pathogenic bacterium
Supervisor
:
Prof. Nabih Abdel Rahman Bashen
Thesis Type
:
Master Thesis
Publishing Year
:
1426 AH
2005 AD
Co-Supervisor
:
Dr. Akram Salah al-Din Ahmed
Added Date
:
Wednesday, June 11, 2008
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
زينة وليد شعراوي
Sharawi, Zeina Walid
Researcher
Master
zsharawi@kau.edu.sa
Files
File Name
Type
Description
23976.pdf
pdf
المستخلص
23977.pdf
pdf
Abstract
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